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Cytokine gene expression and NF‐κB activation following infection of intestinal epithelial cells with Eimeria bovis or Eimeria alabamensis in vitro
Author(s) -
ALCALACANTO Y.,
IBARRAVELARDE F.
Publication year - 2008
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.2007.01015.x
Subject(s) - eimeria , biology , immune system , immunology , cytokine , in vitro , microbiology and biotechnology , biochemistry
SUMMARY In cattle, Eimeria produces clinical disease with different degrees of severity, depending on the dominant species . Eimeria bovis triggers severe intestinal damage, while E. alabamensis causes minimal damage. Cytokines and other factors derived from epithelial cells play important roles in inflammatory and immune responses in intestinal tissue. This study aimed to obtain a detailed view of IFN‐γ and IL‐4 mRNA expression as well as of activation of NF‐κB p50 and p65 subunits induced by E. bovis or E. alabamensis in intestinal epithelial cells by means of a RT‐PCR assay and a NF‐κB p50/p65 ELISA‐based kit, respectively. Our results demonstrate that infection by both Eimeria species enhances IL‐4 mRNA expression in intestinal cells. However, IL‐4 was expressed more intensely in cells incubated with E. bovis whereas IFN‐γ levels were higher and detected at an earlier time in cells infected with E. alabamensis. NF‐κB was activated in infected cells irrespective of species, yet the activity of the p50 subunit was significantly higher in cells incubated with E. bovis. Our results suggest that the intensity of host‐cell responses triggered by these two Eimeria species could be considered as potential determinants of pathogenicity.