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Identification of a 55 kDa Haemonchus contortus excretory/secretory glycoprotein as a neutrophil inhibitory factor
Author(s) -
ANBU K. A.,
JOSHI P.
Publication year - 2008
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.2007.00995.x
Subject(s) - haemonchus contortus , biology , glycoprotein , immune system , parasite hosting , integrin alpha m , flow cytometry , immunology , excretory system , microbiology and biotechnology , helminths , endocrinology , world wide web , computer science
SUMMARY Haemonchus contortus is an economically important gastric parasite infecting sheep and goats. The parasite survives the host immune attack by releasing protective molecules. In the present study, a 55 kDa secretory glycoprotein (gp55) was identified that inhibited host neutrophils as judged by reduced H 2 O 2 production by these cells. The binding of gp55 to neutrophils was confirmed by flow cytometry. This binding was mediated by cellular CD11b/CD18 integrin. The glycoprotein gp55 also bound to goat monocytes and lymphocytes and inhibited monocyte function. Using light fluorescence microscopy, gp55 was localized at the surface of adult worms. The absence of gp55 in the infective L 3 larvae and its expression in the blood‐feeding stage support a role for gp55 in the parasitic stage of the organism.