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Recognition profiles of microsporidian Encephalitozoon cuniculi polar tube protein 1 with human immunoglobulin M antibodies
Author(s) -
FURUYA K.,
OMURA M.,
KUDO S.,
SUGIURA W.,
AZUMA H.
Publication year - 2008
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.2007.00988.x
Subject(s) - encephalitozoon cuniculi , biology , antibody , immunoglobulin m , monoclonal antibody , glycoprotein , antigen , immunology , immunoglobulin g , virology , microbiology and biotechnology , microsporidia , spore
SUMMARY Microsporidian Encephalitozoon cuniculi has a unique organelle called a polar tube (PT), the extrusion of which is absolutely required to invade a host cell. We recently detected anti‐ E. cuniculi PT immunoglobulin (Ig) M antibodies in sera from many healthy individuals. The present one‐dimensional (1‐D) immunoblot analysis predominantly detected a band at 52 kDa in all of the examined human sera with anti‐PT IgM. The use of mouse monoclonal antibody confirmed that the 52‐kDa band detected in 1‐D immunoblots was an antigen derived from the PT, which represents a glycoprotein nature. In addition, from changes in the immunoreactivity of the 52‐kDa band before and after treatment with NaOH, we determined that the 24 human serum samples with anti‐PT IgM activities could be roughly grouped into three types: (i) sera containing antibodies against only a saccharic determinant ( n = 3); (ii) sera containing antibodies against only a proteinic determinant ( n = 11); and (iii) sera showing dual recognition of saccharic and proteinic determinants ( n = 10). Further two‐dimensional (2‐D) immunoblot analysis followed by proteomic analysis confirmed that human sera with anti‐PT IgM reacted with E. cuniculi polar tube protein 1 (PTP1). Such circulating IgM antibodies may be important in the first line of defence against E. cuniculi infection.