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Lipopopeptidephosphoglycan from Entamoeba histolytica activates human macrophages and dendritic cells and reaches their late endosomes
Author(s) -
VIVANCOCID H.,
ALPUCHEARANDA C.,
WONGBAEZA I.,
ROCHARAMÍREZ L. M.,
RIOSSARABIA N.,
ESTRADAGARCIA I.,
VILLASISKEEVER M. A.,
LOPEZMACIAS C.,
ISIBASI A.
Publication year - 2007
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.2007.00963.x
Subject(s) - endosome , entamoeba histolytica , biology , microbiology and biotechnology , immunology , dendritic cell , immune system , intracellular
SUMMARY Lipopopeptidephosphoglycan (LPPG) is a complex macromolecule from the surface of Entamoeba histolytica trophozoites. We analysed the interaction between LPPG and human macrophages and dendritic cells (DCs) and found that LPPG is internalized by these cells and activates them. The internalization process involves intracellular traffic from the cell membrane to late endosomes, as shown by co‐localization of LPPG with late endosomes marked with FITC‐dextran and LAMP‐1. LPPG‐activated DCs have increased expression of co‐stimulatory molecules CD80, CD86 and CD40 and produce pro‐inflammatory cytokines TNF‐α, IL‐8 and IL‐12. Taken together, these results show that LPPG activates antigen‐presenting cells and reaches intracellular compartments that are involved in antigen presentation.