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Immunization with recombinant beta‐tubulin from Trypanosoma evansi induced protection against T. evansi , T. equiperdum and T. b. brucei infection in mice
Author(s) -
LI S.Q.,
FUNG M.C.,
REID S.A.,
INOUE N.,
LUN Z.R.
Publication year - 2007
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.2006.00933.x
Subject(s) - trypanosoma evansi , biology , recombinant dna , virology , trypanosoma brucei , microbiology and biotechnology , trypanosomiasis , gene , genetics
SUMMARY The beta‐tubulin gene of Trypanosoma evansi ( STIB 806) was cloned and expressed in Escherichia coli . The predicted amino acid sequence of T. evansi beta‐tubulin shows 100%, 99·8%, 99·1%, and 98·6% homology with T. equiperdum, T. b. brucei , T. cruzi and T. danilewskyi , respectively, but is diverse from that of T. cyclops , showing only 51·6% of homology. Recombinant beta‐tubulin was expressed as inclusion bodies in E. coli. It was purified and renatured for immunological studies. Mice immunized with the renatured recombinant beta‐tubulin were protected from lethal challenge with T. evansi STIB 806, T. equiperdum STIB 818 and T. b. brucei STIB 940, showing 83·3%, 70% and 76·7% protection, respectively. Serum collected from the rabbit immunized with recombinant beta‐tubulin inhibited the growth of T. evansi, T. equiperdum and T. b. brucei in vitro. Serum from mice and rabbits immunized with recombinant beta‐tubulin recognized only T. evansi beta‐tubulin and not mouse beta‐tubulin. The results of this study demonstrated that the recombinant T. evansi beta‐tubulin is a potential candidate for the development of a vaccine to prevent animal trypanosomiasis caused by these three trypanosome species.