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Cytokine responses in immunized and non‐immunized calves after Ostertagia ostertagi infection
Author(s) -
CLAEREBOUT E.,
VERCAUTEREN I.,
GELDHOF P.,
OLBRECHTS A.,
ZARLENGA D. S.,
GODDEERIS B. M.,
VERCRUYSSE J.
Publication year - 2005
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.2005.00780.x
Subject(s) - ostertagia ostertagi , immunology , cytokine , biology , immune system , lymph , interleukin 4 , interferon gamma , interleukin 10 , helminths , medicine , pathology
SUMMARY The objective of this study was to evaluate abomasal cytokine responses in helminth‐naive calves and calves vaccinated with protective antigen fractions from Ostertagia ostertagi after an experimental challenge infection with infective third stage (L3) larvae . Abomasal lymph nodes and/or abomasal mucosa were collected and messenger RNA for the Th1 cytokines (IFN‐γ, IL‐2, IL‐12 p40 subunit), the Th2 cytokines (IL‐4, IL‐5, IL‐6, IL‐10, IL‐13, IL‐15) and the Th3/Tr cytokine TGF‐β was quantified by real‐time RT‐PCR. Vaccination had no effect on cytokine profiles in either the abomasal lymph nodes or the abomasal mucosa. However, following infection all calves showed a significant decrease in the Th1 cytokines, IFN‐γ and IL‐12 p40, and a significant increase in the Th2 cytokines, IL‐4, IL‐5, IL‐10 and IL‐13 in the lymph nodes, compared to non‐infected calves. No correlation between the Th2 response and protection induced by vaccination could be demonstrated. In contrast, a Th2 pattern was not observed in the mucosa of the infected calves, which exhibited an increase in IFN‐γ as well as in the Th2 cytokines IL‐4, IL‐5 and IL‐10 mRNA. No significant association was observed in the abomasal mucosa between any examined cytokine mRNA level and immune effector responses such as parasite‐specific antibodies or the number of mucosal mast cells or eosinophils.