Premium
Function and expression of a human idiotypic network in Schistosomiasis japonica
Author(s) -
WISNEWSKI ADAM V.,
OLDS G. RICHARD,
JOHNSON JOAN H.,
RAMIREZ BERNADETTE,
KRESINA THOMAS F.
Publication year - 1996
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1996.tb01027.x
Subject(s) - idiotype , biology , polyclonal antibodies , immunology , schistosomiasis , antibody , antigen , immune system , antiserum , schistosoma japonicum , schistosomiasis japonica , immunoglobulin idiotypes , immunosuppression , monoclonal antibody , helminths
SUMMARY The cross‐reactive idiotype (Hu‐SJ‐CRI M ) is defined by polyclonal human anti‐idiotypic antibodies derived from chronically S. japonicum infected patients. The present study shows that serum levels of Hu‐SJ‐CRI M expressed by antibodies to S. japonicum soluble egg antigen (SEA) are associated with acute infection and hepatosplenic disease. Xenogeneic anti‐idiotypic antisera (anti‐Hu‐SJ‐CRI M ) suppressed human lymphocyte blastogenesis to SEA in vitro by 47–82% (P < 005). These anti‐idiotypic antibodies also suppressed in vitro granuloma formation induced by SEA coated beads in a dose dependent manner. This immunosuppression was antigen specific in that mitogen (PHA) or non‐related antigen (PPD) induced blastogenic responses were not suppressed. Surprisingly, anti‐idiotypic antibodies (anti‐SJ‐CRI M ), which describe the mouse correlate CRI M were not suppressive in the human blastogenesis or in vitro granuloma formation assays. These data indicate a dichotomy in the function and specificity of the idiotype/anti‐idiotype human and murine immune networks in S. japonicum infection. Thus, only the patient derived molecules and serology form the basis for an immunoregulatory network in Schistosomiasis japonica.