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Effect of chemotherapy on immune response to egg antigens of Schistosoma mansoni in chronically infected children from areas of low transmission
Author(s) -
NOYA O.,
LOSADA S.,
NOYA B. ALARCÓN DE,
GONZÁLEZ S.,
HERMOSO T.,
BALZAN C.,
CESARI I.M.
Publication year - 1995
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1995.tb01012.x
Subject(s) - schistosomiasis , praziquantel , biology , antigen , schistosoma mansoni , immunology , serology , immune system , transmission (telecommunications) , antibody , precipitin , chemotherapy , helminthiasis , schistosoma , virology , helminths , genetics , electrical engineering , engineering
SUMMARY In an attempt to identify antigenic molecules from Schistosoma mansoni eggs, a serological study was performed on children of a Venezuelan town (Carabal‐leda) in which the transmission of schistosomiasis had been interrupted two years prior to sera sampling. Infected children received treatment with Praziquantel and, based on the disappearance of eggs in the stools plus negativiza‐tion of the circumoval precipitin test (COPT) one year after treatment, they were classified as either responders or non‐responders to chemotherapy. Western blots of soluble egg antigen (SEA) with a very sensitive chemiluminescent substrate were performed. Sera from responder children recognized a 25kDa band of SEA which diminished significantly after treatment. This was less frequent in non‐responder children. When the sera of responder and non‐responder children were compared before treatment, we found that the recognition of the 40 and 41 kDa proteins could be predictive of response to chemotherapy. All these antigens, used in ELISA‐type techniques, might be of importance in the evaluation and follow‐up of large scale schistosome control programmes.