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Immunogenicity of recombinant Plasmodium falciparum rhoptry associated proteins 1 and 2
Author(s) -
STOWERS ANTHONY,
PRESCOTT NICOLE,
COOPER JUAN,
TAKACS BELA,
STUEBER DIETRICH,
KENNEDY PETER,
SAUL ALLAN
Publication year - 1995
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1995.tb01009.x
Subject(s) - rhoptry , immunogenicity , epitope , biology , monoclonal antibody , recombinant dna , plasmodium falciparum , antibody , virology , antigen , antigenicity , fusion protein , immunology , epitope mapping , immunofluorescence , apicomplexa , malaria , gene , biochemistry
SUMMARY Mice and rabbits immunized with recombinant forms of malaria vaccine candidate antigens rhoptry‐associated proteins 1 and 2 (RAP‐1, RAP‐2 and rRAP‐l, rRAP‐2) produce antibodies at litres equivalent to monoclonal antibody ascites fluid raised against the native proteins. Sera from animals immunized with rRAP‐l contain antibodies which recognize the native protein by indirect immunofluorescence and immunoblotting, partially inhibit erythrocyte invasion in vitro and are long lasting. Epitope mapping shows these antibodies predominantly recognize epitopes in the N‐terminal third of rRAP‐l, some of which coincide with the targets of inhibitory monoclonal antibodies. By contrast, sera from animals immunized with rRAP‐2 contain antibodies which recognize the recombinant but not the native protein