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Cytokine profiles for human Vγ9+ T cells stimulated by Plasmodium falciparum
Author(s) -
GOODIER MARTIN R.,
LUNDQVIST CARINA,
HAMMARSTRÖM MARIELOUISE,
TROYEBLOMBERG MARITA,
LANGHORNE JEAN
Publication year - 1995
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1995.tb00909.x
Subject(s) - schizogony , biology , plasmodium falciparum , elispot , antigen , interferon gamma , cytokine , tumor necrosis factor alpha , microbiology and biotechnology , immunology , cytotoxic t cell , interleukin 2 , in vitro , virology , malaria , cd8 , biochemistry
Summary Vγ9+ T cells from malaria non‐exposed donors make proliferative responses to Plasmodium falciparum on in vitro stimulation. Vγ9+ cells are strongly activated by components of the schizont stage of the parasite and by antigens released into the culture upon schizogony, while CD4+ Vγ9 ‐ cells are stimulated by the earlier stages of the parasite. Using reverse transcriptase‐polymerase chain reaction (RT‐PCR) we determined mRNA expression for 14 cytokines in highly purified Vγ9+ cells enriched by positive selection after in vitro stimulation with P. falciparum schizont antigens. Interferon‐γ (IFN‐γ) and Tumor Necrosis Factor‐α (TNF‐α) were detected in all samples tested. The majority of samples also expressed TNF‐β, transforming growth factor‐β (TGF‐β) and Interleukin‐8 (IL‐8). Only occasional samples expressed IL‐2, IL‐5 and IL‐10. Using the ELISPOT assay we found that a large fraction of the reactive Vγ9+ cells produced IFN‐γ and that γδ T cells are the major producers of IFN‐γ in cultures stimulated with schizont antigens. The majority of Vγ9+ cells in these cultures also express the membrane‐bound form of TNF‐α. Expression of these cytokines speaks for a cytolytic and/or inflammatory role of γδ cells in the response to malaria in non‐exposed individuals.