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Cellular and humoral responses to antigenic subunits of Echinococcus granulosus cyst fluid in hydatid patients
Author(s) -
PROFUMO E.,
ORTONA E.,
RIGANO R.,
GIOIA I.,
NOTARGIACOMO S.,
IOPPOLO S.,
SIRACUSANO A.
Publication year - 1994
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1994.tb00366.x
Subject(s) - echinococcus granulosus , antigen , biology , peripheral blood mononuclear cell , immunology , antibody , hydatid cyst , protein subunit , echinococcosis , blot , microbiology and biotechnology , cyst , pathology , in vitro , medicine , biochemistry , zoology , gene
SUMMARY The hydatid fluid antigenic subunits which stimulate the proliferative response of T cells in PBMC isolated by hydatid patients were identified. In the same subjects, the serum IgG antibody profiles were determined by immuno‐blotting to compare T cell activation and IgG production. The study was carried out on 18 patients with clinically and serologically diagnosed hydatidosis and on ten healthy blood donors. To assess the proliferative response to the different antigenic subunits, sheep hydatid fluid was separated by the SDS‐PAGE and the fractions were blotted onto nitrocellulose and solubilized. The comparison between immunoblotting and PBMC proliferation assay shows that the 55 and 65kDa subunits of antigen 5 and the 12kDa subunit of antigen B are the most reactive subunits in the two techniques. The 16 and 20 kDa subunits of antigen B are more reactive in PBMC proliferation assay than in immunoblotting, thus assigning a specific role to antigen B in cellular response.