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Human monocytes cultured with and without interferon‐gamma inhibit Plasmodium falciparum parasite growth in vitro via secretion of reactive nitrogen intermediates
Author(s) -
GYAN BEN,
TROYEBLOMBERG MARITA,
PERLMANN PETER,
BJÖRKMAN ANDERS
Publication year - 1994
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1994.tb00362.x
Subject(s) - plasmodium falciparum , biology , in vitro , parasite hosting , interferon gamma , monocyte , nitric oxide , secretion , incubation , interferon , growth inhibition , immunology , microbiology and biotechnology , biochemistry , malaria , endocrinology , world wide web , computer science
SUMMARY Adherent cells from human peripheral blood were studied for their interaction with asexual blood forms of Plasmodium falciparum in vitro . Freshly isolated monocytes only showed weak anti‐parasitic effects. However, an enhancement of this anti‐parasitic activity was apparent when monocytes were allowed to mature in vitro . Monocytes activated with IFN‐γ for two or three days had an enhanced anti‐parasitic effect. In contrast, the inhibition mediated by cells incubated for five days was the same with or without IFN‐γ treatment. There was no evidence of toxicity when IFN‐γ at high concentrations was added directly to P. falciparum cultures. The anti‐parasitic activity of the activated cells seemed to be due to nitric oxide since incubation of macrophages with L‐NMMA reduced the level of inhibition. However, inhibition was only partial suggesting that other factors also were involved in inhibition of parasite growth.