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Detection of antibodies in sera from Chagas' disease patients using a Trypanosoma cruzi immunodominant recombinant antigen
Author(s) -
PARANHOSBACALLA GLAUCIA S.,
SANTOS MARCIA R. M.,
COTRIM PAULO C.,
RASSI ANIS,
JOLIVET MICHEL,
E.CAMARGO MARIO,
DA SILVEIRA JOSÉ FRANCO
Publication year - 1994
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1994.tb00336.x
Subject(s) - trypanosoma cruzi , chagas disease , recombinant dna , biology , virology , antigen , antibody , immunology , epitope , trypanosomiasis , kinetoplastida , parasite hosting , protozoal disease , malaria , gene , biochemistry , world wide web , computer science
SUMMARY A Trypanosoma cruzi DNA fragment encoding an immunodominant repetitive antigen (H49) was subcloned into a protein purification and expressed system. Purified H49 peptide reacted specifically in an enzyme‐linked immunosorbent assay (ELISA) with sera from T. cruzi ‐infected patients, but not with sera from patients with other parasitic diseases such as leishmaniasis and T. rangeli ‐infection. The H49 recombinant ELISA was able to detect specific antibodies in 84% of chronic chagasic serum samples tested. One of the major advantage of the recombinant ELISA for serodiagnosis of chronic Chagas’disease resides in its high specificity (100%). Our data suggest that recombinant peptides could provide a practical basis for specific diagnosis tests for Chagas’disease.