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The inhibitory effect of ovine recombinant interferon‐gamma on intracellular replication of Toxoplasma gondii
Author(s) -
OURA C. A. L.,
INNES E. A.,
WASTLING J. M.,
ENTRICAN G.,
PANTON W. R. M.
Publication year - 1993
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1993.tb00641.x
Subject(s) - toxoplasma gondii , biology , recombinant dna , interferon gamma , parasite hosting , fibroblast , virology , in vitro , interferon , viral replication , immunology , immune system , virus , antibody , biochemistry , world wide web , computer science , gene
Summary A model for the in vitro infection of ovine cells with Toxoplasma gondii tachyzoites has been developed and used to investigate the effect of treatment with ovine recombinant interferon‐gamma (ov.rIFNγ) on parasite replication. Treatment of both alveolar macrophages and fibroblast cells either 24 h pre‐infection or 2 h post‐infection with ov.rIFNγ inhibited replication of T. gondii and was quantified by suppression of 3 H uracil uptake by the parasite. Replication of T. gondii in the fibroblast cells was significantly inhibited by treatment with 200‐300 U/ml ov.rIFNγ, whereas concentrations as low as 1 U/ml suppressed parasite replication in the alveolar macrophages.