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The effect of irradiation on the third stage larvae of Brugia pahangi
Author(s) -
DEVANEY E.,
BANCROFT A.,
EGAN A.
Publication year - 1993
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1993.tb00627.x
Subject(s) - brugia pahangi , biology , larva , immunology , helminthiasis , helminths , zoology , ecology
Summary The effect of irradiation on the third stage larvae of the filarial nematode Brugia pahangi was investigated. Labelling with 33 S methionine of control or irradiated L 3 , post‐infective L 3 or L 4 revealed no consistent alterations in the pattern of proteins synthesized. The only significant difference observed was in 125 I labelling, where the specific activity of labelling of soluble cuticular proteins was lower in irradiated than in control parasites. This difference may be related to the reduced size of irradiated parasites rather than to a specific effect of irradiation on the expression of cuticular proteins. Irradiated parasites recovered on day 14 post‐infection were significantly shorter than control parasites. Irradiation also appeared to have a lethal effect on male parasites, as no recognizable males were recovered from animals given irradiated L 3 , nor were microfilariae ever observed in these animals. The mechanisms by which irradiation may enhance the immunogenicity of L 3 of filarial nematodes are discussed.