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The immunogenicity of hookworm (Necator americanus) acetylcholinesterase (AChE) in man
Author(s) -
BROWN ALAN,
PRITCHARD DAVID IDRIS
Publication year - 1993
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1993.tb00600.x
Subject(s) - necator americanus , acetylcholinesterase , immunogenicity , biology , blot , antigen , aché , context (archaeology) , esterase , immunology , antiserum , virology , microbiology and biotechnology , biochemistry , enzyme , helminths , paleontology , gene , ascaris lumbricoides
Summary A number of different but complementary approaches have been used to demonstrate the immunogenicity of Necator americanus acetylcholinesterase to infected individuals. Western blotting of parasite somatic extracts with human post‐infection sera and a specific rabbit antiserum to AChE resulted in the development of almost identical antigen‐recognition profiles. AChE‐containing fractions produced by preparative iso‐electric focusing were subsequently shown to be antigenic in ELISA using post‐infection sera. This preliminary data was reinforced by the affinity‐purification of AChE by immobilized post‐infection IgG, and the immunoprecipitation of AChE activity from ES by post‐infection IgG. Finally, AChE purified by affinity chromatography on edrophonium chloride was shown to be antigenic by Western blotting, and in ELISA, against post‐infection sera, although a degree of re‐activity was also seen with normal human sera. This data is discussed in the context of the host‐parasite relationship.