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Human monoclonal antibodies against Plasmodium falciparum : production, stabilization and characterization
Author(s) -
KAMBER MARKUS,
BLACKMAN MICHAEL,
LIN PECKSUN,
BROWN JAMES,
WHITTLE HILTON,
SCHMIDTULLRICH RUPERT
Publication year - 1992
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1992.tb00019.x
Subject(s) - biology , monoclonal antibody , plasmodium falciparum , virology , antigen , antibody , immunology , microbiology and biotechnology , epstein–barr virus , immune system , immunofluorescence , malaria , virus
Summary Nine human monoclonal antibodies (MoAbs) recognizing 7 different antigenic structures of blood‐stages of the human malarial parasite P. falciparum (Pf) were produced by Epstein‐Barr virus transformed B‐cell lines (EBV‐TCL) with or without fusion to the lymphoblastoid cell line KR4. The peripheral blood B‐lymphocytes were obtained from 8 Gambian donors immune to Pf malaria. Two of the EBV‐TCL could be expanded and maintained for more than 6 months but neither one could be cloned. Six additional EBV‐TCL were stabilized after fusion with the KR4 lymphoblastoid cell line. All resulting hybridomas permitted easy cloning. Some of the MoAbs produced distinct fluorescent staining patterns of asexual Pf blood‐stage parasites when using high‐resolution digitized video‐intensified fluorescence microscopy. Antigens on 195 kD and 155 kD proteins were recognized by 3 and 1 MoAb, respectively, using Western blotting and immunoprecipitation techniques.