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Immunochemical localization of major hydatid fluid antigens in protoscoleces and cysts of Echinococcus granulosus from human origin
Author(s) -
SANCHEZ F.,
MARCH F.,
MERCADER M.,
COLL P.,
MUÑOZ C.,
PRATS G.
Publication year - 1991
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1991.tb00554.x
Subject(s) - antigen , echinococcus granulosus , biology , cyst , viral tegument , pathology , antigenicity , antibody , parenchyma , hydatid cyst , immunology , virology , medicine , zoology , botany
Summary Monospecitic rabbit an user a obtained through experimental immunization with previously purified proteins were used in the structural localization of two hydatid fluid antigens, antigen 5 and antigen B, in cyst membranes and protoscoleces of E. granulosus from human origin. The antigen‐antibody reaction was revealed by an avidin‐biotin‐peroxidase technique. Antigen 5 was not evident in the laminated membrane of the cyst wall, but it was associated with the germinal membrane of the cyst wall and brood capsules. The parenchyma of invaginated and evaginated protoscoleces was heavily labelled. The tegument, the calcareous corpuscles, the suckers and the hooks did not contain antigen 5. Degenerated protoscoleces were also labelled. Antigen B localization was essentially identical to antigen 5. but degenerated protoscoleces were not recognized by anti‐antigen B anliserum. Technical aspects and differences with previously published work arc discussed.