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Identification of phosphorylcholine containing antigens of Fasciola hepatica —successful tolerization against this epitope in experimental animals
Author(s) -
SLOAN T.,
DOOGE D.,
JOYCE P.
Publication year - 1991
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1991.tb00296.x
Subject(s) - phosphorylcholine , epitope , biology , antigen , fasciola hepatica , polyclonal antibodies , monoclonal antibody , immunology , virology , antibody , helminths , biochemistry
Summary Phosphorylcholine containing antigens have been identified in the parasite Fasciola hepatica by immunoblotting and ELISA. Immunoblots probed with polyclonal and monoclonal antibodies indicate that the majority of antigens identified in both the immature and mature parasite contain both phosphorylcholine and non‐phosphorylcholine epitopes. One antigen of 58 kDa appears to contain predominantly PC epitopes or at least this epitope is the major one responded to by host animals. Successful immunoiolerization against the epitope PC was achieved by injecting the PC conjugate, ovalbumin PC, into neonatal rats. Immurunolerization against PC resulted in a 25% reduction in worm burden upon subsequent infection with Fasciola hepatica.