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Production of interferons by bovine and ovine cell lines infected with Theileria annulata or Theileria parva
Author(s) -
ENTRICAN G.,
McINNES C.J.,
LOGAN M.,
PRESTON P.M.,
MARTINOD S.,
BROWN C.G.D.
Publication year - 1991
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1991.tb00287.x
Subject(s) - biology , theileria parva , cell culture , virology , theileria , interferon gamma , complementary dna , monoclonal antibody , interferon , microbiology and biotechnology , antibody , immunology , cytokine , parasite hosting , gene , genetics , world wide web , computer science
Summary Three bovine cell lines and four ovine cell lines infected with Theileria parva or Theileria annulata were examined for the production ofinierferon (IFN). Biologically active IFN was detected in the tissue culture supernaiants of four of the cell lines. Only one, a bovine cell line infected with T. parva , produced IFN‐y as measured by specific neutralization with a monoclonal antibody to bovine IFN‐y. This observation was confirmed by analysing RNA from the cell lines on Northern blots using an IFN‐y cDNA probe. The other three cell lines which produced IFN were infected with T. annulata. The IFN produced by those lines was not IFN‐y.