Heterogeneity in the expression of surface‐exposed epitopes among larvae of Ascaris lumbricoides

Summary Quantitative immunofluorescence was used to examine differences in the binding of antibody to the surface of individual living infective stage larvae of Ascaris lumbricoides. Using rabbit antisera, it was first established that larvae cultured for 48 h after artificial hatching were relatively uniform in their levels of antibody binding and in minimal exposure of epitopes expressed by later larval stages. Aliquots from a pool of larvae were probed with serum from individual infected people living in an endemic area of Nigeria. The larvae used were derived from parasites collected in the same geographical area in which serum donors were living. Two principal points emerged. First, serum donors varied considerably in the degree to which their antibody bound to the larvae. Secondly, the binding of antibody from a given donor revealed remarkable heterogeneity in surface epitope expression. Such intra‐specific variability in antigen expression has considerable implications for the development of immunity to parasitic nematodes.