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The expression of the Mr 30 000 antigen in the third stage larvae of Brugia pahangi
Author(s) -
DEVANEY EILEEN,
JECOCK ROWENA M.
Publication year - 1991
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1991.tb00264.x
Subject(s) - brugia pahangi , biology , vertebrate , antigen , larva , parasite hosting , helminthiasis , host (biology) , immunology , cuticle (hair) , microbiology and biotechnology , helminths , zoology , anatomy , ecology , gene , genetics , world wide web , computer science
Summary The expression of the M r 30 000 surface antigen in the third stage larvae (L, 3 ) of Brugia pahangi has been investigated. The antigen could be detected only with great difficulty in the mosquito derived Li externally labelled with 125 I but was more easily labelled in 24 and 48 h post‐infective larvae harvested from the vertebrate host. Labelling of a detergent extract of mosquito derived L 3 with 125 I demonstrated that the Mr 30 000 antigen was indeed present in this life cycle stage, presumably in an internal localization. It seems likely that the M r 30 000 antigen is not fully expressed in the parasite cuticle until after infection of the vertebrate host. The data presented also suggest that there arc major differences in the surface properties of the mosquito derived Li compared to the p.i. L 3 harvested from the vertebrate host.