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Production of tumour necrosis factor during murine cutaneous leishmaniasis
Author(s) -
MOLL HEIDRUN,
BINÖDER KERSTIN,
BOGDAN CHRISTIAN,
SOLBACH WERNER,
RÖLLINGHOFF MARTIN
Publication year - 1990
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1990.tb00983.x
Subject(s) - tumor necrosis factor alpha , biology , immunology , spleen , cutaneous leishmaniasis , leishmania major , leishmania , in vitro , leishmaniasis , necrosis , cytokine , parasite hosting , biochemistry , genetics , world wide web , computer science
Summary We have assessed the role of tumour necrosis factor‐α (TNF) during cutaneous leishmaniasis and demonstrated that significant levels of TNF were released by spleen cells from infected mice after in vitro restimulation with Leishmania major promastigotes. Spleen cells from both genetically resistant and genetically susceptible mice were equally capable of producing TNF. After challenge with bacterial endotoxin, TNF activity could also be demonstrated in the serum of L. major ‐infected mice and the titres correlated with the course of cutaneous disease in susceptible and resistant mice. TNF did not exert a direct leishmanicidal effect in vitro. Furthermore, our study indicated that macrophages are the source of L. major ‐induced TNF activity and that its elicitation is dependent on the presence of T cells. These findings suggest that TNF acts in concert with other cytokines produced during L. major infection and that its role depends on the composition of T cell subsets and cytokines present.