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Three non‐repeated transmission blocking epitopes recognized in the 21 kD surface antigen of zygotes‐ookinetes of Plasmodium berghei
Author(s) -
TIRAWANCHAI NEDNAPIS,
SINDEN ROBERT E.
Publication year - 1990
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1990.tb00979.x
Subject(s) - epitope , plasmodium berghei , monoclonal antibody , biology , antigen , virology , antibody , plasmodium (life cycle) , immunology , epitope mapping , microbiology and biotechnology , parasite hosting , malaria , computer science , world wide web
Summary Two‐site and competitive ELISA have been developed against a surface antigen of zygotes‐ookinetes of Plasmodium berghei using monoclonal antibodies (MoAbs) which block transmission of the parasite to the mosquito. Three such MoAbs have been studied, each of which recognized a protein of an M r 21 kD (Pbs21) using immunoblot techniques. The assays showed that there are at least 3 single B‐cell epitopes expressed in Pbs21. One epitope recognized by MoAb 17.9 is conformation dependent and antibodies bound to it interfered with other MoAbs (12.1 and 13.1) each recognizing a different, apparently linear epitope. Glycosylation might not be relevant to the binding of any of the antibodies tested to their respective epitopes.