Premium
The identification of a species‐specific antigen from Necator americanus
Author(s) -
PRITCHARD D.I.,
McKEAN P.G.,
ROGAN M.T.,
SCHAD G.A.
Publication year - 1990
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1990.tb00953.x
Subject(s) - necator americanus , biology , parasite hosting , antiserum , antigen , blot , immunology , identification (biology) , zoology , ecology , biochemistry , helminths , world wide web , computer science , gene , ascaris lumbricoides
Summary A 17 kD protein of Necator americanus was isolated by SDS‐PAGE and used to raise monospecific antisera in rabbits. ELISA and Western blotting against a range of parasite extracts demonstrated the species specificity of this protein. It is expressed at all stages of the life‐cycle, appears to be accumulated through the larval stages to adulthood, and can be localized in the oesophageal glands and cuticle of the adult parasite. The possible nature and diagnostic potential of this protein is discussed.