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Protective immunization of mice against Schistosoma mansoni with purified adult worm surface membranes
Author(s) -
SMITHERS S. R.,
HACKETT FIONA,
ALI PIRLANTA OMER,
SIMPSON A. J. G.
Publication year - 1989
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1989.tb00669.x
Subject(s) - schistosoma mansoni , antigen , biology , antibody , immunization , saponin , immunology , schistosomiasis , vaccination , immunity , schistosoma , immune system , virology , microbiology and biotechnology , helminths , medicine , pathology , alternative medicine
Summary Immunity to Schistosoma mansoni in the mouse was induced by vaccination with adult worm surface membrane (mb‐S). Of several adjuvants tested, including Freund's, BCG and alum, 50 μ g of saponin per mouse given subcutaneously with the antigen was the easiest to administer, and gave consistent protection, approaching levels usually seen in our mouse model after exposure to irradiated cercariae. An antibody response to the schistosomular surface was detected in mice immunized with mb‐S plus saponin which was predominantly anti‐polypeptide, not anti‐carbohydrate, and thus similar to the antibody response of mice exposed to irradiated cercariae. The level of antibodies to M r 90000 and 38000 schistosomular surface antigens as well as to M r 25000 adult surface membrane antigen was significantly correlated with the presence of protection.