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Identification of protein components of Echinococcus granulosus protoscolex antigens for specific serodiagnosis of E. granulosus infection in dogs
Author(s) -
GASSER ROBIN B.,
LIGHTOWLERS MARSHALL W.,
RICKARD MICHAEL D.
Publication year - 1989
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1989.tb00666.x
Subject(s) - echinococcus granulosus , biology , antigen , excretory system , immunology , recombinant dna , microbiology and biotechnology , biochemistry , anatomy , gene , zoology
Summary Proteins of Echinococcus granulosus protoscolex excretory/secretory or deoxycholate solubilized somatic antigens were radiolabelled with 125 I and immunoprecipitated with sera from dogs naturally or experimentally infected with E. granulosus and various control dog sera. Analysis of immunoprecipitates was performed using one‐ and two–dimensional gel electrophoresis to identify antigenic protein components specific for E. granulosus. Using both electrophoretic techniques, a basic component of M r 27000 and an acidic component of M r 94000 were defined in both excretory /secretory and somatic protoscolex antigens, and were specifically identified by 95% and 62% of 21 sera from E. granulosus‐ infected dogs, respectively. An abundant component of M r 35000 was identified by 100% of these dogs, parts of which were E. granulosus specific. Results of this study should allow identification of specific recombinant antigens for routine serodiagnosis of E. granulosus infection in dogs.