Premium
Lack of interspecies barriers in anti–Id stimulated antibody production against Echinococcus granulosus antigens
Author(s) -
CAROL HERNAN,
HERNANDEZ ANA,
BAZ ADRIANA,
NIETO ALBERTO
Publication year - 1989
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1989.tb00658.x
Subject(s) - echinococcus granulosus , antibody , antigen , polyclonal antibodies , biology , immunology , immunogenicity , primary and secondary antibodies , titer , echinococcosis , idiotype , virology , antibody response , monoclonal antibody , zoology
Summary Polyclonal human anti–hydatid antibodies were affinity purified from a hydatid patient serum and used to produce a rabbit antiidiotype: serum. These anti–Id antibodies cross–reacted in ELISA with sera from 11 of 12 hydatid patients studied and with 13 infected or immunized mice sera. All mice primed and boosted with anti–Id produced anti–hydatid antibodies in the primary response and exhibited an increase in antibody titre after a booster injection. The same effect was observed with mice primed with antigen and boosted with anti–Id, although these mice exhibited higher antibody titres. A significant idiotype repertoire is shared by anti–hydatid antibodies produced by different individuals of the same or different species, and anti–Id raised against those antibodies behave as surrogate antigens producing a normal primary and secondary response in animals of different species from that used to isolate the Id.