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Immunization against Plasmodium falciparum with recombinant polypeptides produced in Escherichia coli
Author(s) -
HOLDER ANTHONY A.,
FREEMAN ROBERT R.,
NICHOLLS STEPHEN C.
Publication year - 1988
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1988.tb00248.x
Subject(s) - recombinant dna , escherichia coli , biology , plasmodium falciparum , antibody , antigen , microbiology and biotechnology , immunization , virology , enterobacteriaceae , immunology , malaria , gene , biochemistry
Summary Two proteins produced in recombinant Escherichia coli and containing ammo acid sequences from the Plasmodium falciparum precursor to major merozoite surface antigens (PMMSA) have been partially purified. These proteins, together with a preparation of merozoites, have been used to immunize animals. The antibody response and the degree of protection were compared. Animals immunized with merozoites produced antibodies reacting with many P. falciparum proteins. whereas a response specific for PMMSA was detected in those receiving the recombinant material. Incomplete protection was conferred to both groups and there was no apparent correlation between antibody levels and protection.