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Echinococcus multilocularis : the non‐specific binding of different species of immunoglobulins to alveolar hydatid cysts grown in vivo and in vitro
Author(s) -
ALKARMI T. O.,
ALSHAKARCHI Z.,
BEHBEHANI K.
Publication year - 1988
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1988.tb00234.x
Subject(s) - antiserum , echinococcus multilocularis , biology , in vitro , fluorescein , in vivo , antibody , microbiology and biotechnology , primary and secondary antibodies , immunology , echinococcosis , fluorescence , biochemistry , zoology , quantum mechanics , physics
Summary Alveolar hydatid cysts (AHC) were isolated from C57BL/6J and BALB/c mice al 8 and 12 weeks post‐infection from subcutaneous and intraperitoneal foci and cultured in in vitro. Freshly isolated as well as in vitro ‐grown cysts were incubated with Fc or F(ab') 2 fragments of human, rabbit, mouse, goat or sheep immunoglobulins, then washed and incubated with a fluorescein‐conjugated rabbit or goat F(ab') 2 fraction of antisera to each of the above primary sera. Significant fluorescence on the surface of AHC was detected when they were incubated with human, rabbit or mouse Fc fractions followed by the addition of goat or rabbit fluorescein‐conjugated antiserum. No fluorescence was detected when the AHC were incubated with the F(ab') 2 fragments and fluorescein‐conjugated antiserum, except when the primary antiserum was mouse F(ab') 2 fractions. Cysts grown in vitro retained their binding ability to human, rabbit or mouse Fc fractions. However, the intensity of fluorescence decreased proportionally with lime. The maximum intensity of staining was observed with small cysts. Large cysts showed diminished fluorescence. The non‐specific binding of human, mouse or rabbit immunoglobulins (Fc fractions) to AHC was also confirmed by rosetting with sheep erythrocytes (SRBC) which suggests that the larvae of Echinococcus multilocularis may utilize the presence of Fc receptors on their surface in order to elude immune destruction by the host.