Premium
Excretory/secretory antigen of Toxocara canis: recognition profiles of polyclonal and larvicidal monoclonal antibodies
Author(s) -
DEL AGUILA C.,
CUELLAR C.,
GUILLÉN J.L.
Publication year - 1988
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1988.tb00217.x
Subject(s) - polyclonal antibodies , toxocara canis , antiserum , biology , monoclonal antibody , antigen , canis , excretory system , microbiology and biotechnology , antibody , monoclonal , in vitro , immunology , endocrinology , biochemistry , ecology
Summary Five monoclonal antibodies against the excretory/secretory (E/S) antigen of Toxocara canis were obtained and characterized. Immunoprecipitating activity was demonstrated in an in‐vitro micropre‐cipitating assay using live T. canis larvae. Their capacity to kill larvae was also shown in an in‐vitro assay. Two zones of reactivity were observed in 7.5 and 12.5% SDS‐PAGE (177‐77 kD, 43‐15 kD) of immunoprecipitates of human and mouse positive polyclonal anlisera. The murine monoclonal antibodies showed a common pattern of reactivity with the proteins in the 177‐77 kD range.