Premium
Impaired natural defence of beige (Chediak‐Higashi syndrome) mice against tissue‐migrating larvae of Strongyloides ratti and its reconstitution by bone marrow cells
Author(s) -
NAWA Y.,
ABE T.,
IMAI J.,
MARUYAMA H.
Publication year - 1988
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1988.tb00208.x
Subject(s) - bone marrow , biology , chédiak–higashi syndrome , immunology , immune system , andrology , medicine
Summary The susceptibility of C57BL/6‐bg J /bg J mice, which exhibit a murine counterpart of the Chediak‐Higashi syndrome, to infection with Strongyloides ratti was examined. After a primary infection, the peak of the daily larval output in faeces (LPG) of bg J bg J mice was approximately twice as high as that of their littermate bg J /+ mice. The total number of tissue migrating larvae recovered from bg J /bg J mice at 36 h after infection was also approximately twice as high as that from bg J +mice. However, after a primary infection, bg J /bg J mice could completely expel adult worms in the intestine by day 14. When an equal number of tissue migrating larvae obtained from the head of +/+ mice were implanted into bg J /bg J and bg J /+ mice, the magnitude and the kinetics of LPG were comparable between them, indicating that in both groups implanted larvae established in the intestine lo become adult worms and then they were expelled by day 13. Thus, immune mechanisms involved in worm expulsion of bg J /bg J mice were comparable to those of bg J /+mice. The higher susceptibility of bg J /bg J mice could be reduced to the level of bg J /+ mice by bone marrow grafting from bg J /+mice 6 weeks prior to infection. Furthermore, when lethally irradiated bg J /bg J mice or bg J /+mice were reconstituted with either type of bone marrow cells, the mice given bg J /bg J bone marrow cells showed higher susceptibility to infection with S. ratti regardless of the genotype of the recipients. These results indicate that the impaired natural defence of bg J /bg J mice is predetermined at the level of haemopoietic stem cells.