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Inhibition of equine neutrophil chemotaxis and chemokinesis by a Taenia taeniaeformis proteinase inhibitor, taeniaestatin
Author(s) -
LEID R. W.,
GRANT R. F.,
SUQUET CHRISTINE M.
Publication year - 1987
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1987.tb00500.x
Subject(s) - chemokinesis , chemotaxis , biology , motility , zymosan , immunology , microbiology and biotechnology , in vitro , biochemistry , receptor
Summary Taeniaestatin, a recently isolated Taenia taeniaeformis proteinase inhibitor, was used to inhibit equine neutrophil migration. Taeniaestatin itself was not chemotactic when used as a chemotactic factor but taeniaestatin did inhibit neutrophil chemokinesis when tested in a Zigmond‐Hirsch checkerboard assay. A dose‐dependent inhibition of both chemokinesis and chemotaxis was observed when zymosan activated bovine sera (ZABS) was used as the chemotactic factor. This inhibition was > 95% when 5 u of taeniaestatin was present on both the cell and chemotactic factor side of the chambers. Equine neutrophils gave dose‐ and time‐dependent migration responses to purified bovine C5a with an ED 50 of 1±04 ± 10 –7 M. Taeniaestatin inhibited the C5a‐mediated chemotactic and chemokinetic neutrophil responses (51% using 1 u and > 95% with 5 u of taeniaestatin). The inhibition of leucocyte motility by taeniaestatin was reversible and without cytotoxicity at the highest doses of taeniaestatin tested.