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The use of aldehydes to show a relationship between host and parasite antigens at the surface of adult male Schistosoma mansoni
Author(s) -
HARNETT W.,
KUSEL J. R.,
BARROWMAN M. M.
Publication year - 1985
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1985.tb00087.x
Subject(s) - schistosoma mansoni , parasite hosting , antigen , biology , host (biology) , glutaraldehyde , trematoda , immunology , schistosoma , incubation , immune system , formaldehyde , microbiology and biotechnology , schistosomiasis , helminths , ecology , biochemistry , pathology , medicine , world wide web , computer science
Summary An indirect radiolabelled antibody method has been developed to measure the effects of aldehydes on the amount of antigen detectable at the surface of adult male Schistosoma mansoni. Incubation of schistosomes in formaldehyde (0·01–10% wt/vol.) or glutaraldehyde (0·01–0·1% wt/vol.) was found to result in increased exposure of parasite antigens with a concomitant decrease in the amount of surface located host RBC antigens. Those concentrations of formaldehyde or glutaraldehyde which were most effective in removing or displacing host antigens were also most able to expose parasite antigens. These findings are consistent with the hypothesis that host antigens mask parasite antigens at the surface of the adult schistosome.