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Changes in the haemolytic activity of bovine serum complement by Hypoderma lineatum (insect oestridae) larval proteinases in naive and immune cattle
Author(s) -
BOULARD CHANTAL,
BENCHARIF FIFI
Publication year - 1984
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1984.tb00816.x
Subject(s) - biology , immune system , hemolymph , complement system , alternative complement pathway , classical complement pathway , larva , enzyme , immunology , parasite hosting , antibody , insect , instar , complement factor b , microbiology and biotechnology , biochemistry , ecology , world wide web , computer science
Summary Three serine proteinases (hypodermin A, B and C) of the first instar larvae of Hypoderma lineatum have been assayed for their ability to deplete seric complement of naive or immune cattle. In naive cattle complement consumption is initiated by hypodermin B through the sequence C1‐C3 at a concentration of 5 pg/ml of serum, and by hypodermin A through the sequence of C3‐C9 at a higher concentration of 150 μg. The third enzyme presenting a collagenolytic activity has no anti‐complementary activity even on C1 q. In immune cattle a 70% complement depletion through the classical pathway is observed with 15 μg of hypodermin B per ml of serum. The two enzymes A and C appear to play a minor role in the complement depletion via the classical pathway. The biological role of each of these enzymes in the parasite‐host interrelationships is discussed. The participation of these enzymes in the immediate hypersensitive reactions following systemic treatment of cattle infested by this endoparasite is considered.