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Activation of complement in C‐reactive protein positive sera by phosphorylcholine‐bearing component isolated from parasite extract
Author(s) -
SUGANE KAZUO,
OSHIMA TOMOO
Publication year - 1983
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1983.tb00754.x
Subject(s) - phosphorylcholine , precipitin , immunoelectrophoresis , coomassie brilliant blue , biology , toxocara canis , polyacrylamide gel electrophoresis , immunodiffusion , microbiology and biotechnology , biochemistry , immunology , antibody , enzyme , helminths , staining , genetics
Summary Phosphorylcholine‐bearing component levels in extracts of various parasites were determined by a capillary precipitin test using anti‐phosphorylcho‐line Ig A myeloma protein, TEPC‐15. Phosphorylcholine was demonstrated as a structural component not only in nematodes but also in trematodes and cestodes. The phosphorylcholine‐bearing component was isolated from an extract of Toxocara canis larvae using a TEPC‐15‐Sepharose 4B column. The component reacted with C‐reactive protein in sera to form one precipitin line in Immunoelectrophoresis. The component provided two Brilliant Coomassie Blue positive bands in SDS‐polyacrylamide gel electrophoresis. It reacted with C‐reactive protein to activate complement in serum.