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Antibody‐dependent cytotoxicity of Trypanosoma cruzi antigen‐coated mouse cell lines by eosinophils and neutrophils
Author(s) -
LÓPEZ A. F.,
SANTOS R. RIBEIRO DOS,
SANDERSON C. J.
Publication year - 1983
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1983.tb00725.x
Subject(s) - trypanosoma cruzi , immunology , biology , cytotoxicity , antigen , antibody dependent cell mediated cytotoxicity , antibody , virology , in vitro , monoclonal antibody , parasite hosting , world wide web , computer science , biochemistry
Summary Eosinophils and neutrophils are shown to be cytotoxic against two syngeneic mouse cell lines cells when these are coated with T. cruzi antigen and anti‐T. cruzi antibody. Activity is detected within 5 h of incubation. Highest levels of cytotoxicity are obtained at antibody dilutions of 1:100 and 1:1000, while antiserum at 1:10 is shown to be inhibitory. Eosinophils show significant activity at an effector to target ratio of 5:1. No cytotoxicity occurs in the absence of either antigen, antibody or effector cells. This phenomenon may be a model for the tissue destruction in acute T. cruzi infection, where the lysis of trypanosomes may lead to antigen coating of host cells, followed by antibody‐dependent granulocyte‐mediated cytotoxicity of the host cells.