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Sj23, the target antigen in Schistosoma japonicum adult worms of an immunodiagnostic hybridoma antibody
Author(s) -
CRUISE KATHY M.,
MITCHELL G. F.,
GARCIA E. G.,
TIU W. U.,
HOCKING ROBYN E.,
ANDE R.F.
Publication year - 1983
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1983.tb00721.x
Subject(s) - schistosoma japonicum , biology , antigen , antibody , epitope , polyacrylamide gel electrophoresis , schistosomiasis , gel electrophoresis , microbiology and biotechnology , virology , immunology , helminths , biochemistry , enzyme
Summary An IgG 2a mouse hybridoma‐derived antibody (designated I.134) has been identified which binds to Schistosoma japonicum adult worms and which has immunodiagnostic potential (for detection of antibody) in schistosomiasis japonica in the Philippines. The target epitope of this hybridoma antibody is contained in a 23 000 molecular weight protein of adult worms as analysed by one‐dimensional sodium dodecyl sulphate‐polyacrylamide gel electrophoresis of immunoprecipitates and a gel overlay technique. This adult worm antigen has been labelled biosynthetically using 35 S‐methionine as well as exogenously using lactoperoxidase‐catalysed radioiodination and the Bolton and Hunter reagent with intact worms. As anticipated, the low molecular weight protein antigen (designated Sj23) appears to be one of several major immunogenic proteins of worms which induce antibodies in infected Philippine patients.