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Immunity to coccidia in chickens: adoptive transfer with peripheral blood lymphocytes and spleen cells
Author(s) -
ROSE M. ELAINE,
HESKETH PATRICIA
Publication year - 1982
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1982.tb00429.x
Subject(s) - coccidia , biology , peripheral blood , spleen , immunity , immunology , adoptive cell transfer , immune system , virology , t cell , parasite hosting , world wide web , computer science
Suspensions of cells prepared from the caecal tonsils and spleen, and the peripheral blood lymphocytes of chickens immune to Eimeria maxima , were tested for their ability to transfer resistance to syngeneic recipients. The intravenous injection of approximately 6 × 10 8 spleen cells or peripheral blood lymphocytes caused a significant reduction of oocyst production by the challenged recipients, in comparison with controls which were uninjected or given cells from birds susceptible to E. maxima. Peripheral blood lymphocytes appeared to be most effective when obtained 10–15 days after a primary, or 3–10 days after a secondary inoculation of oocysts. The peripheral blood lymphocytes which participate in the early response to challenge of immune birds were not found to be protective. When given intraperitoneally, greater numbers of spleen cells were required to reduce oocyst production, and small numbers of caecal tonsil cells were ineffective. The ability of the various cell suspensions to transfer antibody and cell‐mediated responses was monitored with ‘marker’ antigens. There was some indication that both types of response were involved in protection.