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Peanut agglutinin affinity chromatography of Trypanosoma cruzi glycoproteins
Author(s) -
MARCIPAR A. J.,
LENTWOJT E.,
SEGARD E.,
AFCHAIN D.,
FRUIT J.,
CAPRON A.
Publication year - 1982
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1982.tb00424.x
Subject(s) - trypanosoma cruzi , biology , glycoprotein , agglutinin , affinity chromatography , peanut agglutinin , immunology , virology , biochemistry , microbiology and biotechnology , lectin , enzyme , parasite hosting , world wide web , computer science
Summary Glycoproteins from Trypanosoma cruzi epimastigotes have been extracted by diiodosalycilic acid and lithium salts, and phenol‐water biphasic partition. Peanut agglutinin has been used in a one step preparative method for fractionating the total extract in order to separate the so‐called galactose‐terminal glycoproteins. The different fractions have been studied by SDS electrophoresis, ultracentrifugation and immunoelectrophoresis techniques. The experimental immunogenicity, antigenicity and specificity of the PNA affinity fractions has been evaluated.