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The binding of antibodies from Plasmodium berghei ‐infected rats to isoantigenic and parasite‐specific antigenic sites on the surfaces of infected reticulocytes
Author(s) -
BROWN K. N.,
McLAREN DIANE J.,
HILLS L. A.,
JARRA W.
Publication year - 1982
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1982.tb00417.x
Subject(s) - antiserum , biology , plasmodium berghei , antibody , antigen , virology , immune system , ferritin , parasite hosting , microbiology and biotechnology , immunology , malaria , biochemistry , world wide web , computer science
Summary Ferritin‐labelling techniques at the ultrastructural level have shown that antiserum from August rats immune to P. berghei infection contains antibodies which bind to the surfaces of parasitized reticulocytes but not to uninfected cells. Two antibody specificities have been demonstrated by comparing antisera i absorbed with infected reticulocytes, ii absorbed with uninfected reticulocytes, and iii unabsorbed. Ferritin labelling was much increased with antiserum preabsorbed with uninfected reticulocytes, and also with heat‐inactivated serum, indicating a blocking effect on parasite‐specific antibody binding by cold‐reacting anti‐erythrocyte isoantibodies known to be present. Energy‐dependent aggregation, shedding and endocytosis of labelled material was observed at the surfaces of unfixed infected reticulocytes.