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A study of the antigens produced by adult Fasciola hepatica maintained in vitro
Author(s) -
LEHNER R.P.,
SEWELL M.M.H.
Publication year - 1980
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1980.tb00046.x
Subject(s) - fasciola hepatica , biology , antigen , serology , hepatica , in vitro , immunodiffusion , immunology , helminths , antibody , biochemistry
Summary Metabolic antigen of adult Fasciola hepatica was prepared by concentrating the culture medium in which 100 adult flukes had been maintained in vitro for 7 days. Serological reactions between this antigen and sera from fluke‐infected rabbits, rats and sheep were studied using immunodiffusion in agar and enzyme linked immunosorbent assay. The used medium contained a number of antigenic components and their various reactions with the sera from these different hosts were investigated.