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Trypanosoma cruzi : binding of parasite antigens to mammalian cell membranes
Author(s) -
SANTOS R. RIBEIRO DOS,
HUDSON L.
Publication year - 1980
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.1980.tb00039.x
Subject(s) - trypanosoma cruzi , biology , amastigote , antigen , immunofluorescence , parasite hosting , intracellular , cell , microbiology and biotechnology , immunology , antibody , biochemistry , leishmania , world wide web , computer science
Summary Muscle and neuronal cell lines were infected with Trypanosoma cruzi and stained for the presence of parasite antigens by immunofluorescence. Up to 72 h post infection, fluorescence was limited to the intracellular amastigote stages. After the release of parasites, at about 96 h, fluorescence was also associated with the membranes of normal and infected cells. This finding was reproduced by passive sensitization of uninfected cells using amastigote antigens at protein concentrations as low as 10 μg ml ‐1 . Intriguingly, although both normal and transformed cells of muscle and nervous tissue origin adsorbed significant quantities of antigen, lymphocytes and erythrocytes failed to show any detectible uptake.