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Relevance of combinatorial profiles of intermediate filaments and transcription factors for glioma histogenesis
Author(s) -
Colin C.,
Virard I.,
Baeza N.,
Tchoghandjian A.,
Fernandez C.,
Bouvier C.,
Calisti A.,
Tong S.,
Durbec P.,
FigarellaBranger D.
Publication year - 2007
Publication title -
neuropathology and applied neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.538
H-Index - 95
eISSN - 1365-2990
pISSN - 0305-1846
DOI - 10.1111/j.1365-2990.2007.00829.x
Subject(s) - histogenesis , nestin , vimentin , olig2 , intermediate filament , glial fibrillary acidic protein , sox10 , biology , pathology , oligodendroglial tumor , glioma , intermediate filament protein , immunohistochemistry , cancer research , microbiology and biotechnology , transcription factor , oligodendrocyte , oligodendroglioma , neural stem cell , astrocytoma , stem cell , medicine , immunology , central nervous system , gene , neuroscience , cytoskeleton , genetics , myelin , cell
In order to define specific markers for histogenesis of three well‐characterized subgroups of human gliomas (pilocytic astrocytomas, glioblastoma multiforme and oligodendrogliomas), we studied the expression of relevant markers that characterize gliomagenesis, by immunohistochemistry and in situ hybridization. They include the intermediate filament proteins glial fibrillary acidic protein (GFAP), vimentin and nestin, the transcription factors Olig2, Nkx2.2 and Sox10, and the proteolipid protein transcripts plp/dm20 . We show that the three major categories of human gliomas express a combinatorial profile of markers that gives new insights to their histogenesis and may help diagnosis. Pilocytic astrocytomas strongly express GFAP, vimentin, Olig2, Nkx2.2 and Sox10 but not nestin. In contrast, glioblastomas strongly express GFAP, vimentin and nestin but these tumours are heterogeneous regarding the expression of the transcription factors studied. Finally, in oligodendrogliomas, intermediate filament proteins are generally not observed whereas Olig2 was found in almost all tumour cells nuclei while only a subpopulation of tumour cells expressed Nkx2.2 and Sox10.