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Acrylamide and 2,5‐hexanedione induce collapse of neurofilaments in SH‐SY5Y human neuroblastoma cells to form perikaryal inclusion bodies
Author(s) -
Hartley C.L.,
Anderson V.E.R.,
Anderson B.H.,
Robertson J.
Publication year - 1997
Publication title -
neuropathology and applied neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.538
H-Index - 95
eISSN - 1365-2990
pISSN - 0305-1846
DOI - 10.1111/j.1365-2990.1997.tb01310.x
Subject(s) - neurofilament , acrylamide , neuroblastoma , sh sy5y , cytoskeleton , intermediate filament , cell culture , microbiology and biotechnology , chemistry , biology , biophysics , cell , biochemistry , immunology , immunohistochemistry , genetics , organic chemistry , copolymer , polymer
C.L. Hartley, V.E.R. Anderson, B.H. Anderson & J. Robertson (1997) Neuropathology and Applied Neurobiology 23, 364–372 Acrylamide and 2,5‐hexanedione induce collapse of neurofilaments in SH‐SY5Y human neuroblastoma cells to form perikaryal inclusion bodies Neurofilament accumulations are characteristic of a number of neurological conditions including amyotrophic lateral sclerosis, giant axonal neuropathies and several chemically‐induced neuropathies. Although the mechanism(s) leading to neurofilament accumulation are unknown, it is possible that similar processes occur both in disease and in chemically‐induced neuropathies. Understanding the mechanism(s) of chemically‐induced neurofilament accumulation, which is more amenable to experimental manipulation, may give insight into the neurological diseases they mimic. We have compared the effects of two chemically‐dissimilar neurotoxins, 2,5‐hexanedione and acrylamide, on neurofilaments in the human neuroblastoma cell line, SH‐SY5Y. Both undifferentiated and differentiated SH‐SY5Y cells were exposed to 2,5‐hexanedione or acrylamide and changes in cytoskeletal organization examined by immunofluorescence and electron microscopy. Although distinct morphological differences have previously been characterized in the neuropathies induced by 2,5‐hexanedione and acrylamide in vivo , we have found that both compounds had similar direct effects on neurofilaments in SH‐SY5Y cells, inducing formation of perikaryal inclusion bodies. In addition, differentiated SH‐SY5Y cells were more sensitive to both 2,5‐hexanedione and acrylamide compared with undifferentiated cells. These similar effects of 2,5‐hexanedione and acrylamide lend further support that a common mechanism(s) may lead to neurofilament accumulation in these neuropathies. SH‐SY5Y cells provide a useful model to investigate further the biochemical basis of neurofilament accumulation.