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Detection of numerical chromosome aberrations in brain tumours by fluorescence in situ hybridization on smear preparations of small tumour biopsies
Author(s) -
Löw M.,
Feiden W.,
Moringlanet J. R.,
Zang K. D.,
Henn W.
Publication year - 1994
Publication title -
neuropathology and applied neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.538
H-Index - 95
eISSN - 1365-2990
pISSN - 0305-1846
DOI - 10.1111/j.1365-2990.1994.tb00993.x
Subject(s) - fluorescence in situ hybridization , interphase , in situ hybridization , pathology , in situ , biology , chromosome , hybridization probe , fish <actinopterygii> , cytogenetics , microbiology and biotechnology , dna , medicine , gene , genetics , chemistry , gene expression , organic chemistry , fishery
We present a technique for the preparation and interphase cytogenetic analysis of native tumour cell nuclei from smear preparations of small biopsies of human brain tumours. The presence of tumour–specific numerical chromosome aberrations can be determined by fluorescence in situ hybridization (FISH) analysis using chromosome–specific repetitive DNA probes. The FISH analysis of smear preparations provides cytogenetic information about brain tumour samples, within 2 days, avoiding time–consuming and artefact–prone tumour cell culture.