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Expression of glial fibrillary acidic protein (GFAP) by cultured angiofibroma stroma cells from patients with tuberous sclerosis
Author(s) -
KATO M.,
KATSUMOTO T.,
OHNO K.,
KATO S.,
HERZ F.,
TAKESHITA K.
Publication year - 1992
Publication title -
neuropathology and applied neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.538
H-Index - 95
eISSN - 1365-2990
pISSN - 0305-1846
DOI - 10.1111/j.1365-2990.1992.tb00826.x
Subject(s) - glial fibrillary acidic protein , gfap stain , vimentin , pathology , keratin , intermediate filament , biology , tuberous sclerosis , stroma , fibronectin , immunocytochemistry , immunohistochemistry , microbiology and biotechnology , cell , medicine , extracellular matrix , cytoskeleton , genetics
Large dendritic cells were cultured from facial angiofibromas of six patients with tuberous sclerosis. The cells were examined immunocytochemically for expression of selected cyto‐skeletal and non‐structural proteins and the results compared with the staining profiles obtained with normal skin fibroblasts and normal glial cells. In similarity to normal glia, the angiofibroma stroma cells expressed glial fibrillary acidic protein (GFAP). Conversely, by analogy to fibroblasts, the abnormal stroma cells produced fibronectin and did not react with the antibody to S‐100 protein. By immunogold labelling it was established that GFAP and vimentin were co‐localized in intermediate filaments of the angiofibroma cells.