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Investigation of the expression of epidermal growth factor receptor and blood group A antigen in 110 human gliomas
Author(s) -
JONES N. R.,
ROSSI M. L.,
GREGORIOU M.,
HUGHES J. T.
Publication year - 1990
Publication title -
neuropathology and applied neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.538
H-Index - 95
eISSN - 1365-2990
pISSN - 0305-1846
DOI - 10.1111/j.1365-2990.1990.tb01155.x
Subject(s) - epidermal growth factor , antigen , a431 cells , monoclonal antibody , biology , epidermal growth factor receptor , epidermoid carcinoma , antibody , immunohistochemistry , cell culture , receptor , pathology , cancer research , microbiology and biotechnology , immunology , cancer , carcinoma , medicine , molecular medicine , cell cycle , genetics , biochemistry
Investigation of the expression of epidermal growth factor receptor and blood group A antigen in 110 human gliomas. The presence of epidermal growth factor receptor (EGF‐R) and blood group A antigen was studied immunohistochemically in a series of 110 malignant gliomas using monoclonal antibodies. Fifty‐seven percent of the tumours strongly expressed EGF‐R on the malignant cells. Although blood group A antigen is present on EGF‐R of A431 cells (a cell line derived from a human epidermoid carcinoma), in gliomas it was found only on vascular endothelial cells of tumours from blood group A patients. The results suggest that the EGF‐R present in gliomas differs from that in A431 cells in the type or amount of the carbohydrate chains. This is in contrast to previous reports which have suggested that A antigen is present on EGF‐R in gliomas. This has relevance in the choice of monoclonal antibodies used to study the EGF‐R, as those directed against the A antigen component of the A431 cell EGF‐R will not recognize EGF‐R elsewhere and may cause normal blood group A antigen to be mistaken for EGF‐R.