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Immunolocalization of cathepsin D in the human central nervous system and central nervous system neoplasms
Author(s) -
ROBSON D. K.,
IRONSIDE J. W.,
REID W. A.,
BOGUE P. R.
Publication year - 1990
Publication title -
neuropathology and applied neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.538
H-Index - 95
eISSN - 1365-2990
pISSN - 0305-1846
DOI - 10.1111/j.1365-2990.1990.tb00930.x
Subject(s) - choroid plexus , central nervous system , pathology , biology , polyclonal antibodies , cathepsin d , antibody , endocrinology , medicine , immunology , biochemistry , enzyme
The cellular distribution of the lysosomal proteinase cathepsin D was studied in a series of 76 neoplasms and 18 non‐neoplastic tissues from the human central nervous system, using a well‐characterized polyclonal antibody in a peroxidase‐antiper‐oxidase technique. In the normal and developing brain, cathepsin D is confined to neurons and choroid plexus epithelium. Strong granular cytoplasmic staining was present in neuronal and choroid plexus neoplasms, and in reactive macro‐phages. A large variety of other neoplasms also exhibited positive cytoplasmic staining, albeit usually of a weaker diffuse type. Cathepsin D cannot be considered a specific marker for neuronal or choroid plexus neoplasms, but the antiserum used in this study may be of value in antibody panels for the investigation of these tumours. Its localization may also be of value in embryological studies, particularly in the cerebellum, and in investigations of steroid hormone receptor‐associated proteins in meningiomas and Schwannomas.