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RACTERISTICS OF HUMAN MEDULLOBLASTOMA CELL LINE TE‐671 UNDER DIFFERENT GROWTH CONDITIONS IN VITRO: A MORPHOLOGICAL AND IMMUNOHISTOCHEMICAL STUDY
Author(s) -
MØRK S. J.,
MAY E. E.,
PAPASOZOMENOS S. CH.,
VINORES S. A.
Publication year - 1986
Publication title -
neuropathology and applied neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.538
H-Index - 95
eISSN - 1365-2990
pISSN - 0305-1846
DOI - 10.1111/j.1365-2990.1986.tb00140.x
Subject(s) - biology , in vitro , cell culture , microbiology and biotechnology , tubulin , neurofilament , immunohistochemistry , flow cytometry , microtubule , biochemistry , immunology , genetics
Characteristics of human medulloblastoma cell line TE‐671 under different growth conditions in vitro: a morphological and immunohistochemical study The human medulloblastoma cell line, TE‐671, was studied in vitro both in monolayer culture and in a three‐dimensional culture system using gelfoam as the supporting matrix. Flow cytometry studies of cells grown in monolayer culture revealed a unimodal, tetraploid DNA content. Most cells in both in vitro systems contained neuron‐specific enolase (NSE), actin, and tubulin, while only occasional cells or cell clusters contained the 68 000 molecular weight subunit of neurofilaments (NF mol. wt 68 000) or microtubule‐associated protein 2 (MAP‐2). In monolayer culture, long cellular processes containing NSE, NF mol. wt 68 000 and MAP‐2, which were present at 2 days, were nearly absent by 7 days. All antigens were present at 4 days in the organ culture system; by 72 days, cells still stained positively for NF mol. wt 68000 and MAP‐2, but staining for NSE, actin, and β‐tubulin was diminished as compared to 4 days. Retinoic acid (RA) in the 13‐ cis isomer form at 10 ‐6 M was applied to monolayer cultures at day 1 for 6 days and to gelfoam cultures at day 1 for 28 days. RA did not significantly alter cell proliferation up to 7 days in vitro and did not appreciably affect cellular expression of NSE, NF mol. wt 68000, MAP‐2, β‐tubulin, or actin in either system. By electron microscopy, most cells grown under different culture conditions with or without RA treatment appeared to be undifferentiated and polygonal, with occasional cytoplasmic annulate lamellae. The immunohistochemical and ultrastructural features reported indicate that the TE‐671 medulloblastoma line is composed primarily of primitive neuroepithelial cells with a limited potential for neuronal differentiation. This differentiation was not promoted by RA or by an in vitro system known to favour differentiation in a number of human and animal nervous system tumours. The findings suggest that the cells of the TE‐671 line lack either receptors for retinoic acid or the capacity to respond to bound retinoic acid.